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ANTI-ABCA1 Antibody

ABC 1 antibody|ABC Transporter 1 antibody|ABC-1 antibody|ABC1 antibody|ABCA 1 antibody| ABCA1 antibody|ABCA1_HUMAN antibody|ATP binding Cassette 1 antibody|ATP binding cassette sub family A ABC1 member 1 antibody|ATP binding cassette sub family A member 1 antibody|ATP binding Cassette Transporter 1 antibody|ATP-binding cassette 1 antibody|ATP-binding cassette sub-family A member 1 antibody|ATP-binding cassette transporter 1 antibody|CERP antibody|Cholesterol efflux regulatory protein antibody|F

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一抗 - 多克隆抗体

说明书:

BA1541-2

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB

Polyclonal

现货

总价格:

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Product Brief

  • 产品概况

    货号 BA1541-2
    产品名称 ANTI-ABCA1 Antibody
    基因名 ABCA1
    抗体来源 Rabbit
    克隆 Polyclonal
    抗体亚型 Rabbit IgG
    免疫原 A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1 (2231-2261aa KDLSLHKNQTVVDVAVLTSFLQDEKVKESYV), identical to the related mouse sequence.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 Immunogen affinity purified.
    浓度 500ug/ml
    产品形态 Liquid
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 ABCA1 (ATP-binding cassette, sub-family A (ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein (CERP) is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes contain 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.
    研究类别 1. Langmann, T., Klucken, J., Reil, M., Liebisch, G., Luciani, M.-F., Chimini, G., Kaminski, W. E., Schmitz, G. Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages. Biochem. Biophys. Res. Commun. 257: 29-33, 1999.2. Szakacs, G., Langmann, T., Ozvegy, C., Orso, E., Schmitz, G., Varadi, A., Sarkadi, B. Characterization of the ATPase cycle of human ABCA1: implications for its function as a regulator rather than an active transporter. Biochem. Biophys. Res. Commun. 288: 1258-1264, 2001.
    Uniprot ID ABCA1: O95477
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

Instructions

抗体的保存建议:抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)
2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议最少不要少于10ul每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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